How to Analyze Real Time qPCR Data?

qPCR-DAMS: a database tool to analyze, manage, and store both relative and absolute quantitative real-time PCR data.

Quantitative real-time PCR is an important high-throughput method in the biomedical sciences. However, existing software has limitations in handling both relative and absolute quantification. We designed quantitative PCR data analysis and management system (qPCR-DAMS), a database tool based on Access 2003, to deal with such shortcomings by the addition of integrated mathematical procedures. qPC...

How to appropriately analyze ordinal scale data?

How to Analyze Paired Comparison Data

Thurstone’s Law of Comparative Judgment provides a method to convert subjective paired comparisons into one-dimensional quality scores. Applications include judging quality of different image reconstructions, or different products, or different web search results, etc. This tutorial covers the popular Thurstone-Mosteller Case V model and the Bradley-Terry logistic variant. We describe three app...

How to Choose a Normalization Strategy for miRNA Quantitative Real-Time (qPCR) Arrays

Low-density arrays for quantitative real-time PCR (qPCR) are increasingly being used as an experimental technique for miRNA expression profiling. As with gene expression profiling using microarrays, data from such experiments needs effective analysis methods to produce reliable and high-quality results. In the pre-processing of the data, one crucial analysis step is normalization, which aims to...

بررسی اثر گیاه خارخاسک بر نسبت جنسیت اسپرم قوچ عربی خوزستان با تکنیک Real time qPCR

این پژوهش به منظور بررسی اثر گیاه خارخاسک بر نسبت جنسیت اسپرم قوچ عربی خوزستان با تکنیک qPCR Real-time با استفاده از 18 راس قوچ عربی خوزستان با سه تیمار انجام شد. ژنهای SRY و PLP به ‌ترتیب برای جداسازی قطعات خاصی از توالی‌های کروموزوم Y- و X- تکثیر شدند. تیمارهای آزمایشی شامل : 1- گروه شاهد 2- جیره حاوی 15 گرم بر کیلوگرم گیاه خارخاسک 3- جیره حاوی30 گرم بر کیلوگرم گیاه خارخاسک در جیره بود. از تم...